Sentence examples for first necessitated from inspiring English sources

Exact(1)

As the CHS gene sequence has not previously been determined from L. elliptica, this first necessitated PCR amplification of a partial CHS cDNA fragment using degenerate primers followed by cloning and sequencing prior to the development of a RT-qPCR assay for this gene (ii below).

Similar(57)

Two (1.3%) revisions in the second and third postoperative years necessitated component removal (neither were for implant failure), and 7 (4.4%) further secondary operations were required.

Our objective was to investigate changes in total protein as a potential new biomarker of aging, but this necessitated first the development of a fluorescence microscopy method to detect and quantify any changes.

Two weeks after the case, during the weekly Tuesday staff conference at his employers, The Sunday Times, Fleming suffered a serious, second heart attack that necessitated convalescence, which he undertook at the Dudley Hotel in Hove.

Most of these episodes of fever are either non-infectious in origin or related to the diagnosed disease process that necessitated surgery in the first place.

Shortage of paper necessitated rationing to two-thirds of prewar consumption in the case of Britain, while from hundreds of thousands of those in the armed forces came a tremendous demand for light reading.

This necessitated insertion of a second marker within the lesion.

Ultimately, the event demonstrated the limits of what can happen when we try to uproot safe spaces spaces that emerged in a specific geographic and cultural context and transplant them into the wider world, a world that necessitated their existence in the first place.

Second, the population-level approach we adopted necessitated crude measurement of potentially complex decision-making processes that may occur across multiple providers and/or multiple time points [ 21].

Fifteen patients (13.5%) died in the first year, 5 (4.5%) had a complication that necessitated hardware removal and in one patient (0.9%) the final evaluation could not be carried out due to amputation related to diabetes.

Former player Smith, who was the club's director of football, and Russell, the chief operations officer, were the first two high-profile casualties of the cost-cutting necessitated by administration.

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