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Replacement of each base in ACAG of the first motif and CA of the second motif reduced the affinity more than replacement of other bases.
For each pair of motifs (including TATAAA), the upstream regions were divided into four categories: Upstream regions that contained the first motif but not the second, upstream regions that contained the second motif but not the first, upstream regions that contained neither of the two motifs, and upstream regions that contained both motifs (the intersection).
They should only affect this first motif and therefore provide information on whether both or only the first of the putative motifs actively binds CaM.
For each given sequence, the left plot reports the location of the first motif relative to the peakMax on the y-axis, and the location of the second motif relative to the first motif on the x-axis.
Interestingly, one GOBP lacked the fifth motif and one had two copies of the seventh motif.
The region corresponding to the N-terminal sequence obtained from the 29 kD band begins at the third amino acid of the first motif (MMR-A) or at the first amino acid of the first motif (MMR-B).
The results also indicated that AtGRF9 and BrGRF12 contained a second motif 1 downstream of the first.
These interactions may be affected by the third proline mutation P375S in the first PPxY motif of LATS1 and the second proline mutation P516L in the only PPxY motif of LATS2.
A fourth 1-5-10 1-5-10 1-5-10s the first spacer sequence and second IQ motif.
A Gly or Ser residue in MPAM motif (first motif) determines the permeability of K+ or Na+ [ 53].
This DRY motif, located at the junction of the third transmembrane domain and the second internal loop, is the most emblematic motif.
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