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Pre-immune sera were collected 2 weeks (Time -2) prior to the first immunization.
Breast-feeding acts as a baby's first immunization and can prevent 20 percent of neonatal deaths.
The T helper1 (Th1) oriented response persisted for 12 weeks after the first immunization.
Three weeks after the first immunization, BVA (0.25 mg/kg) injected into the Zusanli acupoint (ST36) showed the antinociceptive effect.
The antibody titers were determined after the first immunization.
The first immunization was performed using Complete Freund's Adjuvant (CFA) (Sigma-Aldrich, F5581, St . Louis USA).
Two weeks after the first immunization, the rabbits were boosted with incomplete adjuvant.
Prior to the first immunization, serum was collected from each rabbit to serve as negative control.
The vaccine and dose assigned during the first immunization were maintained for second and third immunizations.
The serum was harvested 8 weeks after the first immunization and purified with an affinity column.
The antibody titers reached peak levels 8 weeks after the first immunization.
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