If this virus were then to infect a second species, the intein could potentially undergo a second homing reaction and become inserted into the homologous gene in the new species.
The first is homing and is initiated by an intron-encoded endonuclease (homing endonuclease gene [HEG]) that recognizes and cleaves an intron-less allele at or near the intron insertion site [reviewed in [ 6]].
Amazon's recommendation engine puts their customers at the center first by homing in on their interests and proclivities.
First, the homing properties of liposomes after intravenous administration of DiI-labeled PSLs were determined by flow cytometry and immunohistochemistry.
Goddard and Burt [ 13] first formulated the homing cycle (Fig. 1) for introns with HE activity; a modified model was applied to inteins [ 3, 14].
Banchereau et al. reported that upon viral infection of humans, different subsets of chemokines are secreted in a temporally regulated manner, with CXCL1, CXCL2, CXCL3 and CXCL16 being secreted first to allow homing of naïve T cells, then CCL3, CCL4, CCL5, CXCL8, CXCL9, CXCL10 and CXCL11 to sustain localization of activated T cells, and finally CCL19, CCL22 and CXCL13 [ 35].
Once pigeons become familiar with their environment, however, they are thought to resort to the second mechanism of homing, involving memorized local features [ 2].
This is the first time an engineered homing endonuclease has been used to modify a chromosomal locus.
We report herein a facile and generic approach to transiently engineer the cell surface using lipid vesicles to present biomolecular ligands that promote cell rolling, one of the first steps in the homing process.
To validate RMS-P3 binding to furin in vivo, we first tested the tumor homing ability of phage RMS-P3/RR to RD, RD-FUR and RD-PDX derived tumors.
