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NGS method supersedes the first generation sequencing method (chain termination method) which was introduced by Frederick Sanger, by incorporating two more dimensions in it viz.
The automated Sanger sequencing approach is frequently referred to as "first generation sequencing".
The automated Sanger method for sequencing is known as first generation sequencing, whereas newer methods developed for sequencing are considered next generation sequencing (NGS) [ 78].
Amosvalidate, which is included as part of the AMOS assembly package (Treangen et al. 2011), was developed specifically for first generation sequencing libraries (Phillippy et al. 2008).
While today, first generation sequencing is not used due to its forbidden cost and time consumption, second generation sequencing technologies are widely used due to their lower cost and time efficiency.
A few years later (2002), the Sanger (dideoxy) first generation sequencing, particularly capillary approaches, allowed the sequencing of the first whole human genome, consisting of about 3 billion base pairs and over 20,000 human genes [ 78, 79].
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Third generation sequencing.
Second generation sequencing.
Genome-wide studies of bacterial gene expression are shifting from microarray technology to second generation sequencing platforms.
In future, novel approaches of sequencing, such as third and fourth generation sequencing can alter the workflow, cost, analysis, and interpretation of genomics associated with PCC/PGL.
We employed hybridization capture methods and second generation sequencing technology to obtain >30 kb of exon sequences from 26 nuclear genes for both H. gigas and Dugong dugon.
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