Exact(5)
For the first five passages, the cells were plated at 500 cells/cm2 and incubated for 3 5 days.
Human neuroblastoma cell U251 cell line was procured from NCI and used for the experiments within the first five passages after revival.
Allantoic fluid and the cell culture supernatants from the first five passages were tested for hemagglutinating activity with a 1% suspension of chicken erythrocytes to exclude avian hemagglutinating viruses (e.g., influenza virus and EDS-76 virus).
HUVEC were used within the first five passages.
The isolated AF cells were cultured in DMEM supplemented with 10 % FBS and 1 % penicillin streptomycin, maintained in a humidified incubator containing 5%% CO2 at 37 °C, and used within the first five passages for in vitro analysis.
Similar(54)
For example, the first two passages above seem to relate memory to acts of speaking and reading.
We observed a significant decrease in the rate of growth (Fig. 1d) and increased cell death (Fig. 1e) in these tumors over the first three passages.
He was hit, he later wrote "by that sweetly ecstatic, rare experience that I have heard described as a 'moment of clarity'". This being the CIA, the solution was kept a secret, but it was solved separately by Jim Gillogly, a California computer scientist, who published the first three passages in 1999.
For the first two passages, there were 10 tasks, five for each.
The first four passages are all neumed in other manuscripts, such as Munich, Bayerische Staatsbibliothek, clm 21562, so these episodes are prominent in the medieval reception of Virgil more broadly.
Cells of the first four passages were used for these studies.
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