Sentence examples for first extension step from inspiring English sources

8) The second primer-extension reaction using the fluorescently labelled 3′- O- 2-cyanoethyl -dNTP derivatives 2– 5 and the reverse transcriptase was carried O- 2-cyanoethyl -dNTPn cO- 2-cyanoethyl -dNTPO- 2-cyanoethyl -dNTPn step (step 2).

This included a 30 seconds denaturation step at 95°C, a 30 seconds annealing step at 55°C (Figα) or 57°C (PAR6 and β-actin), and a 40 seconds extension step at 72°C.

The initial PCR activation step was at 94°C for four minutes, followed by the denaturation step at 94°C for one minute, primer-annealing step at 55°C for 30 seconds, extension step at 72°C for one minute, and the final extension step at 72°C for ten minutes.

Samples were run using the Lightcycler programme, with a 15-minute incubation at 95°C followed by 45 thermal cycles, consisting of a 15-second denaturation step at 94°C, then a 20-second annealing step at 60°C, and a 20-second extension step at 72°C after which fluorescence was read.

PCR was performed at 55 degrees Celsius, 32 amplification cycles, 60 seconds extension step.

The Tc_CLB1 amplicon of 3 kb size uses a 45 seconds extension step, in comparison with the other amplicons that uses just 10 seconds.

PCR was performed for 40 cycles comprising a 60-second denaturation step at 94°C, a 60-second annealing step at 55°C, a 90-second extension step at 72°C followed by 10 minutes at 72°C.

After 6-minute initial denaturation at 96°C, a "hot start" amplification was initiated by adding DreamTaq DNA polymerase, followed by 19 cycles consisting of a 15 seconds denaturation step at 95°C, a 30 seconds annealing step at 64°C, and a 15 seconds extension step at 72°C, with a final extension for 45 minutes at 72°C.

Quantitative PCR was performed with the following programme: pre-incubation at 50°C for 2 minutes, initial denaturation at 95°C for 5 minutes, 40 cycles of denaturation at 95°C for 10 seconds, annealing at 60°C for 30 seconds, elongation at 72°C for 30 seconds, and a 20-second final extension step.

Real-time PCR reactions were performed at 95°C for 15 minutes, followed by 40 cycles of amplification consisting of denaturation step at 95°C for 15 seconds and extension step at 60°C for 1 minute.

PCR cycling conditions were: 2-minute 94°C initial denaturation, 35 cycles of 20-second denaturation, 20-second 57°C annealing, and 45-second 68 72°C extension steps, followed by a final 10-minute final extension.