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The plates were incubated with diluted serum (first dilution of 1 20 followed by serial 3-fold dilutions) for 2 h at room temperature.
This study demonstrates two key results: first, dilution of chlorhexidine correlates directly with its bactericidal activity; second, its effectiveness is directly related to its contact time.
Employing this factor, an absolute 10Be/9Be ratio of 1.464 ± 0.014 was determined for a first dilution of the 10Be-rich master solution.
The first dilution enabled the direct injection of complex samples with minimal pretreatment, and the second dilution realized direct introduction of large volume of strong eluent into the UHPLC column without causing peak broadening or distortion.
Inhibition could be resolved by neutralizing with endotoxin negative (<0.25EU/mL) 0.25N Tris-base at first dilution.
Where: N = total viable colony counts; ∑C = sum of colonies counted from all plates; n1 = number of plates counted at first dilution; n2 = number of plates at second dilution; C = number of colonies counted; d = dilution factor from which the first counts obtained (least counted dilution).
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The curves for Kit A displayed some inconstant results for the first dilutions.
DEM was diluted first to 1 m in ethanol before a second dilution in the agarose solution.
Reproducibility falls off for the fourth dilution due to low or no of signal.
A similar study of 60 volunteers last year showed that 95percentt of those given full-strength vaccine were effectively immunized, compared with 70percentt of those given the one-fifth dilution.
Mass flow controllers were used to control medical grade compressed air flowing through the permeation tube and a second dilution stream, introduced after the permeation tube, to produce concentrations of 101, 199, 484 and 924 ppb of ethanol based on the calibrated permeation tube emission rate.
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