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The first depletion allowance in the United States, called the "discovery depletion," was enacted in 1918 to stimulate oil production for World War I (even though the war had just ended).
First, depletion of Drosophila ATL causes ER fragmentation (Orso et al., 2009), but depletion of human ATL does not.
Moreover, thanks to this first depletion, we aimed at loading into the ProteoPrep20 column a larger amount of LAPs compared to the undepleted plasma.
The first depletion was performed two days before tumour cell challenge, and then every 4 5 days until day 50 after challenge.
First, depletion of cholesterol-containing lipid rafts could facilitate phagocytosis of hamster-derived amastigotes through a pathway that allows slow killing.
The first depletion was done -5 days before ocular infection and this was followed by five additional depletions on days −2, +1, +4, +7, and +10 PI as we described previously [70].
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After the second depletion, more than 93% of NK cells were depleted from the spleens as we described previously [70].
The efficiency of CD4+, CD8+, and CD25+ depletion was monitored by FACS analysis of splenocytes 24 h after the second depletion and before ocular infection.
After the second depletion, more than 95% of CD4+ T cells, CD8+ T cells, or CD25+ T cells were depleted from the spleens as we described previously [72].
Third, depletion of tissue macrophages using ClodroLip significantly reduces peri-tumoral lymphatic vessel density, demonstrating a functional role of macrophages in tumor lymphangiogenesis.
Third, depletion of hCdc14B by siRNA led to hyperphosphorylation of Cdc25, increased Cdk1 activity, mitotic defects such as delayed anaphase chromosomes and multipolar spindles, and finally to mitotic arrest and cell death.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com