Sentence examples for first confluence from inspiring English sources

The nine ccRCC primary cultures established from surgical tissue specimens reached the first confluence (p1) in 8.0 ± 2.1 days.

The acquisition of these alterations de novo during in vitro growth is unlikely since cultures were analyzed at first confluence.

Primary cultures reached their first confluence from around 80% in about 2 weeks and about 10 cell doublings.

Although proliferation was better induced by gelatin rather than fibronectin culture condition, the latter composition allowed PBMCs to be more viable up to the first confluence.

For flow cytometry analysis, cells at the first confluence (p1) were detached from plates with 0.25% trypsin-EDTA (Sigma-Aldrich), rinsed and incubated for 15 min in PBS with 5% FCS.

The genomic studies were performed on all ccRCC primary cultures at first confluence (p1); in addition, for 80MLa and 81BPG cases also cultures at second confluence (p2) or at second (p2) and third (p3) confluences were characterized.

In particular, 80MLa and 81BPG cultures reached the second confluence (p2) after 13 days, and 81BPG culture reached the third confluence (p3) after 20 days.

Moreover, their DNA profile remained stable for at least 3 weeks, till to third confluence.

They grew well till reaching the fourth confluence, and then began to slow down their growth rate.

Moreover, the genomic analysis of some cultures at second and third confluences provided evidence that these cultures did not accumulate further alterations during passages and remained stable for at least 3 weeks.

Additionally, we performed the whole-genome DNA profiling of 80MLa culture also at second (p2) confluence (see Additional File 2) and of 81BPG culture at second (p2) and third (p3) confluences.