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Conditioned media (CM) was first concentrated for 30 min at 3000 rpm using Amicon Ulita-5 centrifugal filter device (Millipore, Billerica US) as described by the manufacturer.
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To determine the optimal time window, we first concentrated on eye size for simplicity.
For gelatin gels, supernatants were first concentrated as discussed above.
He first concentrated on flowerpots, he said.
Again, first concentrating on profits, unsustainable timber extraction for five years followed by 20 years of ecotourism results in the highest NPV profit ($991 ha−1), even more than a scenario of unsustainable timber extraction followed by agriculture and ranching ($933 ha−1).
First, concentrate during an introduction.
First, concentrate on scientific gadgetry.
Only rudimentary initial training was undertaken before elements of the division began the embarkation process in May and June 1916 as they were moved to the United Kingdom, where the individual sub units concentrated for the first time, received arms and other equipment and began the task of undertaking further training at Lark Hill, on Salisbury Plain.
Gel purified cDNA was amplified via PCR (denaturation at 94°C, 30 s; annealing using temperature gradient from 47°C to 50°C, 2 min; extension at 72°C, 3 min; 20 cycles) and the PCR product was pooled and concentrated for the first round denaturation/reassociation step (1 ug PCR product in 50 ul reassociation buffer containing 0.3 M Sodium Phosphate, 0.4 M EDTA, 0.04% SDS, pH 6.8).
Energy extinguishes after being concentrated for a finite time, The Second Law of Thermodynamics.
The first is the ease with which a mineral can be separated from the gangue and concentrated for smelting.
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