Briefly, 10 μl of protein extract was first assayed with 50 μl of firefly luciferase substrate (Promega, Madison, WI, USA) in a luminometer for 10 s.
and confirmed by a second assay with Uni-Gold (Trinity Biotech PK, Ireland), according to the national protocol at the time.
As the variegated phenotype of maternally inherited Dp(1 f LJ9 in the presence of mutant dCTCF is skewed toward fully pigmented eyes, a second assay with the following variegation classes was performed: >80% pigmentation, 80-90% pigmentation, 90-100% pigmentation, and 100% pigmentation.
First, assay sensitivity with respect to DSN in cycle 1 was confirmed by comparing XM02 versus placebo.
Highly significant differences (P < 0.01) were obtained when comparing the first and second assays with the respective baselines.
For example, if an extract contains both HS6ST1 and GalNAc4S6S, we can first assay HS6ST1 activity with heparan sulfate as the acceptor, and then assay GalNAc4S6S activity with chondroitin sulfate A as the acceptor.
Specimens reactive on the first assay were retested with a rapid assay, and results were interpreted as HIV-positive only if both tests were reactive.
A first assay was performed with cloned P1, P2 and F1 plants.
In the first assay, proteins complexed with CDDP-modified oligonucleotide and produced two retarded bands, B1 and B2.
In the first assay, the percentage of seedlings with green cotyledons was scored from seeds germinated on MS/G plates containing 1.0 µM ABA for 11 days.
