The second assay of the diet with MBP/exo (Figure 5) showed that, after eggs eclosion in the first days, the H. hampei larvae were active and feeding.
In a second assay of leptin sensitivity, we next tested whether leptin could stimulate acute phosphorylation of STAT3 in neurons of the mediobasal hypothalamus among the four groups of mice[10], [13], [38].
In these experiments, we first assay for the distribution of UNC-40 GFP UNC-40 GFPorsalongnthel axis (Fig. 3G).
Thus, we first assayed for the presence of phosphorylated RLC in dividing Drosophila S2 cells by staining these cells with an antibody specific for T20S21-phosphorylated RLC [24].
In the first assay, the effect of wogonin on CDK9 activity was examined by monitoring the levels of [P]-incorporation into the phosphorylated substrate peptide.
In the first assay, the percentage of seedlings with green cotyledons was scored from seeds germinated on MS/G plates containing 1.0 µM ABA for 11 days.
As a second assay for the efficiency of autophagy induction, we tested the viability of yeast cells producing the long transcript when placed under autophagy-inducing conditions.
In a first assay, SLBs were composed of 99.4% POPC, 0.5% biotin-cap-DOPE, and 0.1% Texas Red-DHPE.
On the other hand, six cases (n° 8, 18, 20, 22, 23) showed valid conversion after the first assay and invalid control of conversion at the second analysis.
The seeds used in the assay of second year (year 2011) were from self-pollinated plants of the first assay (year 2010).
