Sentence examples for first antibody of from inspiring English sources

After blocking with 20% Blocking Reagent N102, the membrane was treated with first antibody of interest, followed by treatment with biotin-conjugated secondary antibody.

The sections were incubated with the first antibody of rabbit anti-BDNF (1 200, Beijing Biosynthesis Biotechnology Co., Ltd, Beijing, China) or rabbit anti-Aβ antibody (1 400, Cell Signaling Technology, Inc. Danvers, MA, USA) followed by the anti-rabbit Cy3 tagged secondary antibodies (1 200; CWBIO, Beijing, China).

And then PTC-Arg/Au@Gra complexes were further used as multifunctional nanocarriers for the absorption of the second antibody of alpha-fetoprotein (Ab2), which formed sandwich-type immunoassay format through associating with alpha-fetoprotein (AFP) and the first antibody (Ab1).

The second antibody of each antibody pair was biotinylated.

Western blotting analysis was carried out essentially as described previously [ 17] except for two reagents: the second antibody of Clean-Blot (Thermo Fisher Scientific) for guinea pig anti-BCNT-C antibody and the substrate of the nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) solution (Roche) or its tablets (Sigma) for development.

After fixation and permeabilization using the kit (ADG), cells were stained with primary antibody of anti-mouse E2A/Pbx1 and anti-rabbit LC3 or Ub for 30 min, and second antibody of PE anti-mouse and FITC anti-rabbit IgG for 30 min, then cells were explored to ImageStreamX Mark II (Amnis, Merck Millipore, Seattle, WA, USA) for image flow cytometry.

The membrane was washed thoroughly in running distilled water and then rocked in 3 × 10 ml of milk-free TBST buffer with each rinse for 10 min. Then, the membrane was exposed to the second antibody of goat anti-guinea pig IgG conjugated with horse radish peroxidase (Sigma) (1 2000 dilution in 10 ml TBST) for 3 h.

PBS was used as the first antibody for negative control of EZH2 IHC staining (Supplementary Figure 1(c)).

The weak signal obtained could result from a partial display of the recombinant protein or from a weak recognition by the first antibody because of unspecific interaction with the second antibody.

First, antibody ligation of the T cell receptor is not physiological, and second, the delineation of human lymphocytes into Th1 and Th2 subsets is considerably less precise than that of the mouse.

For PARP, the first antibody was of Rabbit origin (Ozyme) 1/1000 in TBS 5% milk.