Sentence examples for first and third passages from inspiring English sources

Cells were divided when confluent and experiments were performed only between the first and third passages.

Data measurements are conducted in the second and third passages (includes the hub turn regions).

In contrast, PGI2 production was higher at the first two cell passages, with a significant decrease at the third passage (6.46 ± 0.10, 5.98 ± 0.08, and 1.62 ± 0.05 ng/mL of supernatant for the first, second, and third passages, respectively), whereas von Willebrand expression was similar among the three cell passages analyzed in this work (64.12%, 66.66%, 65.93% of positive cells, respectively).

However, the cells from the second and third passages entered the lag phase two days later.

Incubation periods for Chinese hamsters were still decreasing between second and third passages.

Additionally, no genomic difference was visible between viruses in the second and third passages in MDCK cells.

The viral materials from the cell cultures as well as the fecal specimens in 20% suspensions, were used for the second and third passages.

To determine whether incubation period differences for each species resulted from differing infectivity concentration in the inocula, we used endpoint titration to determine the brain titers after first, second, and third passages (Table).

The supernatant of brain homogenate was used to inoculate C6/36 cells, and CPE was visible at ≈96 hpi for the first passage and at 72 hpi for second and third passages.

The supernatants of the second and third passages in MDCK cells and the allantoic fluids of the inoculated chicken eggs were individually harvested and processed for full genome sequencing.

Only cells between the first and fourth passages were used for experiments.