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This was evident in a significant peak of combined first and second instar An.
We performed herbivory bioassays using first and second instar generalist Lepidoptera larvae Spodoptera littoralis on Arabidopsis thaliana engineered to overexpress novel glucosinolates.
Groups of 50 individuals of first and second instar larvae were placed in glass bottles with purified water, maintained at 26 ± 2° C with a natural photoperiod and supplied with 3 1 powdered mixture of dog food and baking powder.
By using a quantitative ELISA, we demonstrated that ladybird larvae contained 0.7 and 0.5 μg Cry1Ab/g fresh weight in the first and second instar, respectively, when fed continuously with Bt maize-reared spider mites.
Both alleles were lethal in first and second instar larvae, before larval PNS is fully developed.
First and second instar larvae of P. downsi were used for antigen extraction.
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For the herbivory treatments, three second-and third-instar larvae were placed on leaves of an Arabidopsis plant (non bolting).
The abdomen of Protura undergoes anamorphosis: in the first and second instars it has 9 segments, the third 10, and the rest 12. Collembola have a maximum of six abdominal segments.
The level of expression of the M. persicae RyR gene was measured in different developmental stages of clone 4106A, including a mixed pool of first and second instars, mixed third and fourth instars and apterous and winged adults, but no statistically significant differences were found (Fig. 4).
First and second instars of C. montrouzieri were maintained in groups of ten in each Petri dish; from the third instar on, each predator larva was isolated in an individual Petri dish.
funestus representing multiple developmental stages and both sexes: first and third instar larvae, early and late pupae, and male and female adults.
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first and second time
first and fourth instar
first and second level
first and third instar
first and second rally
first and second call
1st and second instar
first and second phase
first and second grade
first and second age
first and second moment
first and second nobody
first and second meeting
first and second book
first and last instar
first and second second-generation
first and second place
first and second second-order
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