Our group was the first to use albumen (i.e. egg white) as a foaming agent [3].
The experimental solutions were given in ovo by injection into the albumen (at two-thirds of the egg's height from the blunt end) using a sterile 1-ml insulin syringe.
Early traits included Early Albumen Height (EAH, mm), First 3 Egg Weight (E3, gr), Early Egg Weight (EEW, gr), Early Production (EPD, % egg production), Early Shell Quality (EPS, gr in pin point pressure), Sexual Maturity (SM, day) and Early Yolk Weight (EYW, gr).
For Pax7 immunostaining, fresh sections were fixed in 4% paraformaldehyde for 20 min, permeabilized with methanol (-20°C) for 6 min, and blocked first with a solution containing 4% bovine serum albumen (BSA; Jackson) in PBS and then with goat anti-mouse AffiniPure Fab fragment (1 100; Jackson) after antigen retrieval with 100 mmol/L sodium citrate.
The thick albumen was poured into the shell first, followed by the embryo on the yolk.
No significant difference in dopamine quantity was found when the brain and the albumen gland of randomly mating, virgin and first time mated snails were compared.
The content of dopamine in the CNS and the albumen gland of randomly mating, virgin and first time mated snails is summarised in Figure 12.
The CNS and the albumen gland of randomly mating, virgin and first time mated snails were dissected in HEPES-buffered Helisoma saline then placed in 50 μl HPLC buffer (see later) and kept on ice.
In addition, the dopamine concentration in both the CNS and the albumen gland of randomly mating, virgin and first time mated animals (Fig. 12) did not differ significantly between experimental groups.
The range of periods and subjects in this sale is vast, from nineteenth-century images (such as Carleton E. Watkins's albumen prints made in the wilds of Oregon in 1867) and modernist compositions (such as Imogen Cunningham's "False Hellebore") to fashion plates by Avedon, unforgiving portraits by Diane Arbus, and a sexy biker girl by Richard Prince.
