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While plmTr is suitable for the FIRMA analysis, plmEx is well suited for probeset-level analysis.
We have further challenged these observations using the robust FIRMA analysis approach [ 42].
Although there was no overlap between the specific genes identified by the SI and the FIRMA analysis methods, we noted that there was an overlap in terms of gene families between the two lists.
FIRMA analysis of the post-DBS ON- stimulus state as compared with the pre-DBS state detected 110 AS events in 106 transcripts (Additional file 8: Table S8), and 8 of these were also identified by the SI method as subjected to DBS-induced AS changes.
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Another extension of the workflow could include a general analysis strategy of the FIRMA scores, which in this study was tailor-made for a two-treatment scenario.
Functional analysis revealed that the FIRMA-detected genes were highly enriched with alternative splicing.
Back on terra firma, we offer up two pages of analysis and contextual reporting on the war in Syria, which has driven so much despair and prompted the largest mass movement of people in decades.
For each sample, 284,258 probe sets belonging to the 'core' set of probes targeting well annotated exons were analyzed, using an annotation file custom-made for alternative splicing analysis with the Finding Isoforms using Robust Multichip Analysis (FIRMA) algorithm (HuEx-1_0-st-v2,coreR3,A20071112,EP.cdf) [ 50, 51].
For SSCP analysis, 0.2 μl of [α 32P] dATP (10 mCi/ml, ~3000 Ci/mmol, FIRMA) was added.
Tévez is on terra firma.
There is no terra firma.
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