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WT and Kcnma1−/− SFR recordings suggest that the combination of losing ∼30% of the rhythmic firing patterns and changing the parameters of the residually rhythmic activity (decreased circadian variation in firing rate, slightly lengthened period, and increased variability of the daily firing peak) is enough to produce a phenotype in circadian behavioral rhythms.
Briefly, we transformed a multi-cell spike pattern into a long sequence, according to each neuron's firing peak times.
Prolonged OHN stimulation evoked two firing phases in HANs: a fast transient firing peak (similar to short stimulation, Figure 1E) followed by slow firing escalation overlapping in time with OHN stimulation.
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Whole cell current clamp recordings of large LNv verify that spontaneous action potential firing peaks in the morning with firing rate gradually decreasing between ZT 0 12 (slope = −0.19) and increasing between ZT 13 24 (slope = 0.07, Figure 1, right panel).
The noise subpopulation was removed from the fire peak subpopulation by applying a threshold corresponding to the 99th percentile of the noise distribution.
Thus, an early dry season fire peak characterizes the west while a late dry season fire peak characterizes the east.
Following the protocol outlined by Foster and Wilson (2007), we found the set of all spike phases for each cell when the rat was at each position and analyzed events defined as 40 ms windows around firing rate peaks.
For clarity, we subtracted 10 from the resulting values, and thus no fast inward rectification is set to 0. Firing threshold, time to peak, half width, and amplitude (measured from firing threshold to peak) of action potentials were analyzed at action potentials evoked at rheobase.
The remaining peak component was composed of two Gaussian subpopulations: (1) noise inherent to statistical analysis and part of the background noise that would not have been removed previously, and (2) fire peaks, each one representing the occurrence of one or more local fire events (Gavin et al. 2006).
The number of flowers produced by plants growing back from fire peaks two or three years afterwards.
However, there were no significant differences in the offset peak firing rates across different durations of the shake events (P>0.05; one-way ANOVA).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com