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Nowadays, the combustion technology must adopt the environment NOx emissions regulations while achieving maximum firing efficiency and performance.
However, the replication and/or firing efficiency of the individual origins on the genomic scale remain unclear.
The firing efficiency at various origins is estimated based on the fold-enrichment of HU-induced ssDNA.
Given that firing efficiency at origins in general is low in S. pombe [9], it is possible that those overlapping origins represent a subset of inefficient origins.
The replication efficiency ηr of the loci at or near the origin sites is the maximal estimate of the firing efficiency ηf of the origins.
Estimation of firing efficiency by the fold-enrichment of ssDNA has been further extended to the whole genome [21], although a small number of late-firing origins have been found to exhibit a high magnitude of ssDNA formation [6], [19].
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In agreement with this, the efficiency of Cdc45 recruitment to yeast origins/MCMs correlates directly with their firing efficiencies [22].
To test whether overlapping origins were inefficient, we performed comparison analysis of the firing efficiencies between the two origin-subsets: the wild type-unique and the overlapping origins.
Therefore there remains a need to develop a method to directly determine the firing efficiencies of origins of DNA replication on a genomic scale.
We estimated the average firing timings T50 and the (maximum) firing efficiencies ηf at the predicted origins using the average replication timing T50 and replication efficiency ηr of the peak loci (Figure 5A).
Yet little is known about the genetic determinants of origin replication times or firing efficiencies.
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