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The typical activity pattern of the culture takes the form of synchronized bursting events (SBEs), in which a large fraction of the recorded neurons simultaneously fire trains of action potentials in short bursts of several hundreds of a millisecond.
To address this question, we stimulated presynaptic pyramidal neurons to fire trains of 15 APs at different frequencies (40, 60, 80, 100 Hz) and simultaneously recorded inhibitory responses in postsynaptic pyramidal cells (Fig. 2a, b).
Recall, type I and type II neurons are able to fire trains of action potentials (tonic firing) if depolarised sufficiently strong which distinguishes them from type III neurons.
As shown here, the neurons we derived could fire trains of APs not before day 37 (~5 weeks) of IVND.
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