Intriguingly, the endogenous expression of both genes increases during finite passaging of primary keratinocytes in vitro.
Since SORBS2-2 and TLR3 can induce senescence after ectopic expression in primary keratinocytes we were curious to evaluate the endogenous expression of these genes during finite passaging of keratinocytes.
In the case of a finite domain, following passage of an invasive activity front, the network settles into a nonzero stable steady state, whose spatial structure will depend on the boundary conditions.
Cultured primary urothelial cells are highly differentiated [10], but demonstrate phenotypic variability between passages [16], [17], and undergo senescence after a finite number of passages [18], [19].
Using this procedure, we observed that most human oral epithelial cells can be expanded for a finite number of passages, achieving a replicative senescent state after approximately 55 days, but few cultures escaped cell senescence, and instead became immortal (G.L. Sanchez, K.L, R.C., J.S.G et al., manuscript in preparation).
Due to a limitation of primary cell cultures (finite number of passages), experiments were primarily done as biological replicates rather than technical replicates.
In an effort to do so, going beyond the Debye-Hückel limit, we first derive an expression for streaming potential by invoking the consequences of strong EDL (electrical double layer) interactions in the narrow fluidic passage and finite conductance of the Stern layer.
A finite element software package like PASSAGE is a valuable tool that will aid greatly in the implementation of warm forming as a manufacturing process beyond the scope of the laboratory and into production.
This work introduces a new thermal finite element analysis software called PASSAGE®/Forming (PASSAGE) that enables the up-front design of the thermal management of warm forming dies.
Like normal human epithelial cells, HRBECs display a finite life and senesce after three passages, providing a limited amount of starting experimental material.
In addition to these cell lines, early passage primary cultures of finite-lifespan HRBECs from other donors were tested whenever sufficient cell numbers were available for the assays employed in this study.
