The other six cp genomes had four to nine gaps, which were then finished gap closure by PCR-based sequencing.
DR directed the sequence finishing and gap closure activities.
KKS conducted genome assembly, sequence finishing and gap closure activities, and created the final assembly.
After sequence assembly, finishing of gaps were performed by sequencing of PCR products with primers designed on sequencing flanking the gaps, until one single contig was built.
For finishing, the gaps were closed by custom primer walks or PCR amplification, followed by sequencing.
"I finished my gap year with memories to keep me warm in the deep of winter and returned focused and ready to be an adult".
Whether or not his insights into place amount to much more than a hill of beans with a flag stuck in it remains to be seen, because in completing his journey home this promising writer has also finished his gap years; now he must get on with the hard graft of lifelong alienation.
By the time they were finished the gap at the top had come back down to four points, Gabriel Jesus had fully justified Guardiola's decision to keep him in the starting lineup and the goalkeeper, Ederson, had been impudent enough to stroll out of City's penalty area and nonchalantly start to stroke around the ball in midfield.
After finished the gap closing work, we annotated the mitochondrial genomes of BPH and SBPH.
Gone are the days of monumental mechanical calamities, finger-sized fit-and-finish gaps, uneven trim, and overall shoddy workmanship.
Quality means everything from poor fit and finish, gaps between exterior and interior parts, hard plastic that looks cheap and transmissions that break down at 50,000 miles.
