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The wild type cells and cdc10-2E cells finished replication within 60 minutes after release from HU block.
This result suggests that the failure of His C mutant embryos to upregulate string after they finished replication in S15 is not simply a consequence of their extended S phase but rather due to a surveillance mechanism that blocks the G2/M transition because chromatin assembly is not completed.
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The detection of FtsZ in a significant higher abundance in the C2 subpopulation, which is preparing for division after finishing replication, is in agreement with its assigned function as a proposed diffusible factor (Teather et al. [1974]) initiating cell division (Chien et al. [2012]).
A reasonable explanation for these observations is that the 10 µM IPTG-dependent expression of mutant RNR proteins provides a dNTP pool that is too small for the cells to be able to finish replication after rifampicin-treatment when all mRNA synthesis stops, whereas 30 µM IPTG is sufficient to provide nucleotides also for replication run-out.
The study proposes that fragility of FRA3B in lymphoblastoid cells, but not in fibroblasts, is not due to fork slowing or stalling but to a scarcity of initiation events, which forces forks coming from flanking regions to cover long distances to finish replication.
Egress was assessed by light microscopy and calculated as a fraction of schizonts that finished the replication cycle and released merozoites during the incubation time.
In mature pollen of C. mackenii, the generative cells have 2C of DNA content (Hirano and Hoshino 2010 a ), indicating that the generative cells have already finished DNA replication toward PMII at the stage.
Stationary phase liquid cultures were treated for 2 h with 20 μl rifampicin (50 mg/ml dissolved in DMSO) before harvest to finish ongoing replication rounds without initiating new ones.
They all started, and mostly finished, the first replication of DNA (Fig. 2E).
The data do demonstrate that when replication has finished clusters are giving way to dispersed couples.
Centromere coupling is detected as soon as chromosome replication is finished and persists while the recombination protein Dmc1 is loaded onto the chromosomes, suggesting that centromere coupling persists through the time of double strand break formation.
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