Sentence examples for finger engineering from inspiring English sources
This engineering strategy is described, placing particular emphasis on methodological aspects, in order to illustrate the increase in the speed and throughput of zinc finger engineering.
In this report, we used the recently described OPEN zinc finger engineering method to rapidly generate ZFNs that can modify endogenous zebrafish gene targets with high efficiency.
(Others have also noted the relatively greater toxicity of the kdr ZFNs compared with the golden and ntl ZFNs made using the Sangamo BioSciences zinc finger engineering technology [29], [45] although we note that those comparisons were made between experiments which used different ZFN expression vectors).
'MAX' randomization saturates codons located in isolated positions within a protein, as might be required in enzyme engineering, or else on one face of an α-helix, as in zinc-finger engineering.
This chapter illustrates the use of a new, rapid, and widely applicable zinc finger-engineering strategy that produces three-finger domains that bind a wide variety of DNA sequences.
The Zinc Finger Consortium, a group of academic laboratories committed to the development of engineered zinc finger technology, recently described the first rapid, highly effective, and publicly available method for engineering zinc finger arrays.
Sander, J. D. et al. Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA).
Given that a multitude of functional diversities is noted with three-finger toxins, protein engineering in highly variable regions without distorting the three-finger scaffold may result in the development biopharmaceutical agents with novel functions of scientific and therapeutic interest.
Engineering zinc finger protein motifs for specific DNA targets in genomes is critical in the field of genome engineering.
The reagents we used to construct and express ZFNs in our experiments are part of the OPEN Zinc Finger Consortium platform for engineering zinc finger arrays.
The full impact of the fundamental advances generated by the modular assembly approach to engineering zinc finger proteins has only begun to be realized.
