Just over a year ago a set of gene editing techniques zinc finger nucleases, transcription activator-like effector nucleases and engineered meganucleases were chosen as the method of the year for 2011 by the journal Nature Methods (Baker, 2012).
Here, we report the use of zinc-finger nuclease (ZFN) genome editing technology to create Mgat1 disrupted Chinese hamster ovary (CHO) cell lines.
Using this reasoning, we generated AaegTRPA1 -/- mutants using zinc-finger nuclease-mediated genome editing.
These studies evaluate CD4+ T cells modified using zinc finger nucleases to edit the CCR5 gene [ 16] and successful impact of this gene-therapy approach has been recently announced [ 46].
Production of transgenic lines with stably integrated luciferase cassettes in the pfelo1 locus was attempted equipping the luciferase cassettes with pfelo1 homology regions for Zinc Finger Nuclease (ZFN -mediated genome editing.
We have all become used to talking with our fingers through e-mail and editing our responses before we hit Send.
His combination of cinéma vérité camera moves with a jazzy, finger-popping editing scheme and his way of layering images over music anticipate MTV, but his pictures are as memorable for their density of information as they are for their elegant, improvisational sense of style.
Since real-time PCR analysis showed that the loss of KREPA3 or mutation of its zinc fingers blocks editing of multiple mRNAs, we explored whether this could be related to the production of the gRNAs.
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S. & Gregory, P. D. Genome editing with engineered zinc finger nucleases.
The Shangri-Las' finger-snapping is edited to singular effect, as sudden as gunfire.
