This class of mutants is presumably defective in more subtle aspects of wall assembly and highlights the need for finer assays to detect defects in spore wall structure.
To evaluate whether the increase in rf is coordinated with the selection of new combinations of alleles of relevant tolerance genes, these results should be complemented with fine-scale assays of recombination landscapes and genome scanning for footprints of selection.
However, we have extended the conclusions of Matsubayashi et al with a finer temporal assay of rates of wound healing, and show that although ERK1/2 inhibition does reduce the rate of cell migration during healing, ERK1/2 phosphorylation is primarily required for the rapid response to wounding within the first 2 hours.
ACPA fine specificity ELISA assays using peptides derived from citrullinated vimentin, fibrinogen and α-enolase were performed as described previously [ 39, 51].
Primers for SNPs within the Rhg1/Rfs2 locus on Lg G were used in fine melt curve assays as described previously [ 69] with the following modifications.
Future studies, including fine mapping, functional assay, and a replication study with large sample sizes from diverse ethnic populations, are needed to validate our results.
The spatial layout of the MZ is important because it obviates the need for fine markers to assay differentiation states – distance to the distal end is a reliable differentiation marker – and because it makes it straightforward to assay the proliferative contribution to the tissue of all cell subpopulations.
Using the same assay, the fine specificities of these responses were mapped to two previously described[54], [55] although relatively uncommon, minimal epitopes (Fig. 4B), which were confirmed in a 51Cr-release assay on murine and human target cell lines expressing the HLA-A*0201 HLA-A*0201
This study aimed to further investigate these underlying associations in cases of child and adult severe asthma followed by fine-mapping and functional assays (Fig. 1).
VDR, CYP27B1 and all SNPs used for finer mapping were assayed with the Sequenom MassArray platform [7].
