Our non-radiolabeled dye study also supported these findings (data not shown).
Non-parametric tests yielded very similar findings (data not presented).
Immunostaining for parvalbumin, another marker for Purkinje cells, corroborated these findings (data not shown).
An earlier time point at 15 days provided similar findings (data now shown).
A sensitivity analysis revealed that this did not bias our findings (data not shown).
Prenecropsy quantitative angiograms revealed changes in the arterial lumen sizes consistent with these findings (data not shown).
Additional experiments with another mitochondrial ROS indicator, dihydrorhodamine123 (DHR123) (green), further verified these findings (data not shown).
The result showed that the mutants were missing many neurons, consistent with previous findings (data not shown).
Adjustment for trunk fat mass, instead of total fat mass, did not alter the findings (data not shown).
Inclusion of computer / game pad use as additional explanatory variable did not considerably change the main findings (data not shown).
A modified ITT analysis excluding three participants with untreated or inadequately treated GC at baseline resulted in similar findings (data not shown).
