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The supposed finding of a plasmid in R. bellii str.
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The unexpected finding of a C. pecorum 7.5 kbp plasmid (p Cpec) in the L1 genome prompted us to re-investigate the presence of the plasmid in other sequenced livestock and koala C. pecorum, using the raw reads available in our collection [ 1, 2].
Despite the finding of plasmid free cells, these results show stable inheritance without selective pressure and indicate a parasitic lifestyle of the plasmids.
Construction of a Plasmid Containing the Gene Encoding His6-TaGene Encoding
As judged by genetic selection for a plasmid-borne marker, a mutation in phage gene uvsX or uvsY essentially blocked the integration of a plasmid with homology to the T4 genome but no phage replication origin (non-origin plasmid).
Introduction of a plasmid containing AQP9 reverses the metalloid tolerance of the deletion strain.
Omission of a plasmid is indicated.
The finding of a pheromone binding protein and the structure of the replication module suggested that pLG2 is a pheromone responding plasmid.
The finding of a Lu.
The recent finding of plasmids in C. botulinum serotypes A and B housing BoNT/A, BoNT/B or both BoNT/A and BoNT/B genes [17], [24] prompted surveys of serotype B strains [18], [19] and dual neurotoxin C. botulinum strains producing subtypes Bf, Af and Ab BoNTs [6], [18].
The problem of plasmid stability and strain reversion in recombinant cultures is investigated through a complete stability analysis of a plasmid-bearing, plasmid-free mixed culture growing in a chemostat.
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