A comprehensive study from clinical, epidemiologic, and entomologic perspectives was conducted at those health areas where case-patients were found; a second serum sample was collected 2 3 weeks after illness onset to demonstrate the antibody seroconversion or a 4-fold increase in antibody titer (12 ).
A good reproducibility was found when using a second sample, resulting in −97 kJ/mol.
The mutations found were confirmed in a second sample through another PCR reaction with the use of Platinum® Taq DNA Polymerase High Fidelity (Invitrogen, São Paulo, Brazil), followed by sequencing.
A previously published duplication affecting the DYS570 and DYS576 markers [10] was found a second time in a German sample from our study.
For 16 of the patients, a second sample which was taken one hour after the first one was available, providing the opportunity to test whether the components found in the first sample were also present in the second sample, an indication of experimental reproducibility.
A second sample of 98 female subjects was subsequently and independently recruited to play the dictator game and was genotyped for the three significant SNPs found in the first sample.
Finally, for the AIM-InDel marker rs34122827, we found a third allelic state in one Mayan sample (#Marco_03).
Her lawyers reportedly said Wang Lijun took two blood samples yet found no traces of poison, while a third sample that he took – tested only four months later – found low levels of a toxin, apparently insufficient to kill.
For these components found in both samples, we calculated the fraction of reads found in the first sample that were also found in the second sample, obtaining an average over all components of 81.57% (S.E.M. = 18.20).
Find a sample of work from the first seven immortalised in POBA's Art Lives collection.
