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Nuclear sequences were examined using GeneJockey to find a restriction site that would distinguish them using Polymerase Chain Reaction-Restriction Fragment Length PCR RFLPhism (PCR RFLP).
However, we could not find a restriction enzyme with a single cut site for the sites A, D, H, and J within the PCR-1 products.
In cases where a primer set that only amplifies one target cannot be identified, it is possible to use sequence information gathered while testing the primers to find a restriction enzyme that digests only one homeolog or paralog thus eliminating amplification from the corresponding template DNA.
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Yet it may be impossible to find a unique restriction site near the nuclease target sequence.
Justice Kennedy had only once before voted to find an abortion restriction unconstitutional, in Planned Parenthood v. Casey in 1992, when he joined Justices Sandra Day O'Connor and David H. Souter to save the core of Roe v. Wade, the 1973 decision that established a constitutional right to abortion.
Looking up either when you're walking or crawling can be an issue (Site #2).", "I find a lot of restriction in the shoulders- overhead work especially with the SCBA (Site #1)." In addition, some firefighters pointed out that the stiff and oversized neckline of the turnout coat is an additional reason for their limited neck mobility.
It's a safe bet, however, that people who are self-important enough to use a cellphone in a theater would find a way around the restriction in no time.
In order to find a unique solution one restriction needs to be imposed.
They can likely find a way around these restrictions to permit KKR to announce such a transaction at the time of withdrawal, if it chooses this route.
If wildlife officials find a suspicious fish kill, restrictions on water use may well be imposed even before laboratory tests confirm the presence of Pfiesteria.
Mr. Putin had talked extensively before his arrival about his willingness to make compromises that might amend the ABM Treaty, or at least find a way around the restrictions that prevent the United States from proceeding with tests of its antimissile systems.
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