Exact(2)
To analyze the underlying cellular mechanisms of adaptation to ischemia-induced apoptosis through short acidic pretreatment, i.e. acidic preconditioning (APC), Wistar rat coronary endothelial cells (EC) were exposed for 40 min to acidosis (pH 6.4) followed by a 14 h recovery period (pH 7.4) and finally treated for 2 h with simulated in vitro ischemia (glucose-free anoxia at pH 6.4).
Afterwards, cells were washed twice in serum free medium, starved for 24 h in serum free medium and finally treated for 12 and 24 h with 10 ng ml−1 TGF-β1 (R&D, Wiesbaden, Germany) or medium alone (Geng et al, 1999).
Similar(58)
Embedded tissue sections were incubated in histolene for 10 min to remove the paraffin, rehydrated in decreasing concentrations of alcohol (100, 90, 80, 70, and 50% ethanol for 10 min each), and finally treated with dH2O for 10 min.
Between 100 and 200 mg of plant and soil was first reacted with hydrogen peroxide (H2O2) for 24 hours at ambient temperature and further digested in HNO3 + HF, for 36 hours at 80°C, then in HCl for 36 hours at 80°C, and finally treated with HCl-HNO3 for 36 hours at 80°C.
We finally treated mother and child for the skin lesions simultaneously as mentioned before.
The cells were starved with 0.5% FBS culture media for 1 day when they reached 50 60% confluency and were then serum-depleted overnight, treated with or without inhibitors, and finally treated with 250 ng/mL Angptl3 for an appropriate time before immunofluorescent staining, western blot, or G-LISA assay.
Finally, treated clinoptilolite (sodium-clinoptilolite) was dried at 85 °C for 5 h [25].
All were finally treated successfully at National Jewish Hospital, considered the leading medical center for mycobacterial infections.
The samples were finally treated with RNase-free DNAse I (Invitrogen, Basel, Switzerland) for 30 min at 37°C.
A numerical example is finally treated.
The Audio Card finally treats music as a distinct content type meant for background play.
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