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Finally, the libraries were enriched using 15 cycles of PCR and purified by the QIAquick PCR purification kit (Qiagen).
Finally, GO annotations were enriched using Annex and Interproscan data.
These finished libraries were enriched using the Nimblegen SeqCap kit.
Lymphocytes and single cells were enriched using light-scatter.
Phosphopeptides were enriched using TiO2.
Finally, the data were analyzed using the 2−ΔΔCt method.
Finally, the data were analyzed using RotorGene software v5.0 (Corbett Research).
Finally, the data was analysed using Lasergene DNASTAR® software (DNASTAR , Inc.
Biological pathways enriched in the data were identified using IPA software (Ingenuity Systems, Redwood City, CA).
Binders were finally enriched using one additional standard selection round.
Finally, the size-selected cDNA libraries ligated to the Illumina sequencing adaptors were selectively enriched using 15 cycles of PCR, and validated using a Bio-Rad Experion system.
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