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Finally, the cells were analyzed using cell Quest software (BD Biosciences, San Jose, CA).
Finally, the cells were analyzed using a FACS Calibur cytometer (BD).
Finally, the cells were analyzed using FACScan (BD Biosciences, USA) flow cytometer.
Finally, the cells were analyzed using a confocal fluorescence microscope (Olympus FV-1000; sOlympus, Olympus FluoView; Olympus, Center Valley, PA, USA).
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Finally, samples (60,000 cells) were analyzed using a MACSQuant (Miltenyi) flow cytometer, with a 488 nm laser to excite SYTOX Green and a bandpass filter 500 550 nm to detect fluorescence.
Finally, the fluorescence intensities of the stained cells were analyzed using a FACScalibur Flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).
Cells were analyzed using the FACS Aria System and FACSDivaTM Software BDD).
Stained cells were analyzed using the BD FACSAria Cell Sorter.
Cells were analyzed using FACSCantoII (BD Biosciences and BDD FACSDiva software (BD Biosciences).
Cells were analyzed using a FACSCalibur flow cytometer (BD Biosciences) equipped with CellQuest ProTM software.
At 24 h post-transfection, cells were analyzed using a luciferase assay kit (Promega).
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