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Finally, samples were alkylated with a final concentration of 5 mM idoacetamide (IAA; Sigma-Aldrich).
Finally, samples were centrifuged for 5 min at 2500 rpm.
Finally, samples were cured for 10 min at 200 °C.
Finally samples of oil/fat obtained from both methods were analysed by Gas liquid chromatography.
Finally, samples were placed in the vacuum oven for 24 h.
Finally, samples are characterized by a millimetric dark brown slip. 4.
Finally, samples were examined with a Hitachi-7650 transmission electron microscope.
Finally, samples were mounted on a stage for examination using carbon tape.
Then hot and cold wash were given and finally samples were dried.
Finally, samples were shaped into a desired size for further measurements.
Finally, samples Ou 4, Ou 6 and Ou 8 were judged virgin olive oils or EVOOs according to the labs.
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