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The expression of the MotPS stator was induced from an IPTG-inducible Pgrac promoter by addition of 0.1 mM IPTG, followed by incubation at 37 °C for 30 min and finally rotation measurements at 23 °C by tracking the position of 1.0-μm beads attached to the partially sheared sticky filament in motility buffer containing 1.0 mM IPTG, 200 mM NaCl and 10% Ficoll.
Mesiodistal tipping showed the most predictability, at 82.5% with respect to the ideal; this was followed by vestibulolingual tipping (72.9%) and finally rotation (66.8%).
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On day 2, the cover slip was removed and the slide washed five times (two times rotating in washing solution for 5 minutes, two times rotating in sterile water for 5 minutes, and finally one rotation in filtered Milli Q water for 5 minutes).
Finally, this rotation allows the jaw positions to be determined using vertical and horizontal projection profiles.
Finally, the rotation speed and lifter shape are synthetically evaluated from the aspect of wear and energy utilization.
Finally, the rotation and translation matrix are estimated by the quaternion, and an iterative algorithm is used to improve the registration accuracy.
Finally, the rotation vector q α is associated to the grid point p i.
Finally factor rotation is done to facilitate interpretation by providing a simpler factor structure (Zeng and Rasmussen 2005).
Finally, the rotation of the proximal humerus in relation to the diaphysis is corrected to adjust the medial bicipital gutter to the medial insertion of pectoralis major fibers.
Finally, external rotation was used to neutralize the medially directed contraction force of the subscapularis and the pectoralis major muscle [20].
Finally, DNA rotation is hindered and the recognition process begins.
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CEO of Professional Science Editing for Scientists @ prosciediting.com