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The residue was finally reconstituted with 200 μL of the desorbing solvent.

The ethanol solution was finally reconstituted with saline solution (9 mL).

MGM was finally reconstituted with new owners.

After a 10 min centrifugation, pellets were finally reconstituted in 500 μl of 0.1 M NaOH and transferred into scintillation vials.

Digests were cleaned using OMIX C18 SPE cartridges (Agilent, Palo Alto, CA) using the manufacturer's protocol and eluted in 25 µl of 60/40/0.1% ACN/H2O/TFA, dried to completion in the speed-vac, and finally reconstituted in 100 µL of 0.1% formic acid.

After irradiation, the radioactive foil was dissolved with 0.4 mL of freshly prepared aqua regia (HCl/HNO3 = 3/1, v/v) under heating at 90 °C for 5 min. The solution was then dried by heating at 100 °C, reconstituted in 0.4 mL of 0.05 M HCl twice, and finally reconstituted in 0.1 M HCl for shipping.

During the reconstruction phase, normalized data is first restored and a scaling procedure which is exactly the inverse to the normalization is applied to finally reconstitute the data in its original form.

Finally, we reconstituted cyclin D1 polyubiquitination in vitro using purified E1 and E2 enzymes (Fig. 3G).