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Of the remainder, 189 proteins had no contradictory calls in the positive prediction for SP. Another 272 went through further confirmation, of which 182 of these were confirmed to be non-SP and 63 were SP. Of the rest, 27 of them could not be finalized due to lack of ortholog matches in the NCBI database and lack of conserved domain for reference.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com