Sentence examples for final wash before from inspiring English sources

DAPI was added to the final wash before mounting.

DAPI (4',6-Diamidino-2-phenylindole dihydrochloride; Sigma) was included in the final wash before cover-slipping with GelMount (Biomeda).

Hoechst nuclear stain was added in one of the final washes before mounting the cover slips on slides using Hydromount.

Sections underwent final washes before being mounted in Citifluor AF-1 and incubated for at least 24 h in the dark at 20 °C before imaging or storage at 4 °C.

The cells were then washed in 1% BSA, with a final wash in PBS, before mounting on coverslips with mounting media (Dako Inc .. Cells were imaged on a Zeiss 510 confocal microscope using an oil immersion 63X objective with a NA of 1.4.

All washes were in PBS except for final wash with water before mounting coverslips.

Staining for the proliferation marker Ki67 (MIB1 antibody) was performed on cells fixed in 3.7% formaldehyde/0.15 M NaCl for ten minutes, five minutes in 100% ethanol with a final wash in PBS before assay.

A final wash step was performed before incubation with NiDAB SK-41000 substrate kit; Vector Labs) for visualisation.

Finally, the cells were incubated for 5 min in IB prewarmed to 37°C before a final wash into ice cold IB.

After six washes with PBS containing 0.1% (w/v) dodecyl-maltoside (final concentration), a final wash with PBS was used before eluting the immunoprecipitates with 2× SDS- Laemmli sample buffer.

protected from light, the slides were repeatedly washed with PBS, then incubated in 10 mM acetate buffer, pH 6, containing 1 mM CuSO4 for 10 min before a final wash in PBS.