The final trees were visualised with FigTree 1.4.0 [ 108].
The final complexes were visualised by enhanced chemiluminescence autoradiography (Perkin Elmer, Cambridge, MA, USA).
The final products were visualised using the 3-amino-9-ethylcarbazole (AEC) substrate system (DAKO Co., Carpinteria, CA, USA).
The prototype has interfaces to assign intensities and then visualise the final rankings.
After final washing, proteins were visualised with a chemiluminescence detection system (Western Lightning™ Chemiluminescense Reagent Plus, PerkinElmer Life Science, Boston, MA, USA) and with subsequent exposure to an Imager System (INTAS, Göttingen, Germany).
So when I first saw Antonioni's film Zabriskie Point with the explosion scene at the end, in which objects (such as furniture, clothes, a TV set) are levitating with Pink Floyd's music progressively taking over and then Roger Water's final scream, I suppose that visualised a familiar state of mind for me.
Thresholded images were subjected to image calculation procedures designed to highlight image elements (pixels) containing (green) fluorescence associated with the tracer protein; all other sources of fluorescence were visualised in the final images only if no green fluorescence was detectable in that pixel.
The final volumes and fitting were visualised using UCSF Chimera (Goddard et al, 2007).
Six pieces of information per patient can be easily visualised: initial and final values for three variables on a 3D graph, and further variables such as group membership (e.g. male vs. female) can be encoded by different types of vectors, for example vectors of different colour.
Final, quantity assessment was performed by visualising a box plot of the normalised data together with the generation of MA plots for the average data from each dose (Additional file 11).
