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Finally, the entropies of all blocks constitute the dimensional components of the final vector of the sequence.
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To construct the final feature vector of a sequence, the mean value of the features extracted from all frames of the sequence is computed.
The final vectors containing the reporter were sequenced on both strands to verify the correct orientation of the responsive elements, minimal promoter, fluorescent protein coding sequence and SV40 poly-A.
The resulting fluc-pIRES-RLuc8 sequence was cloned into the pLENTI6/V-5 TOPO vector per the manufacturer's instructions to create the final vector necessary for the reverse RBS.
The DNA sequence of the final vector was determined to confirm its structure (Macrogen, Cambridge, MA).
However, the final vectors did not contain the lacO sequence, see Discussion section for details.
Vector clip uses the vector-primer data to identify and mark sequencing vector at both ends of the sequence.
PCR products were sequenced and the correct orientation of the fragment into the final vector was verified by restriction.
The final vector containing a diphtheria toxin cassette (MC1-DTA) permitting negative selection was verified by sequencing.
Finally, the final clean sequence is obtained by trimming off both low-quality regions and vector fragments from the sequence fragment between the two termini of the best reasonable pair.
2. The collars (user specified regions of the vector adapters) chosen by the user on file upload are used for a final vector screening in an attempt to ensure all vector is removed from the sequence before submission to the species databases.
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