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Although many non-5' associated CGIs overlap with repeats [ 18, 19], many do not [ 18, 20], but instead are frequently positioned 3' to known genes, overlapping with final transcribed exons [ 3, 20].
The final transcribed audio recorded data and field notes were translated into English.
After preparing the final transcribed draft, paste the entire text alongside the blog post, article, or show notes.
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DNA-free RNA (1 μg) was mixed with 50 ng of random primers (Invitrogen) in 20 μL of final volume and reverse transcribed to total cDNA with SuperScript II reverse transcriptase (Invitrogen, USA) following the manufacturer's instructions.
After the vote, each senatus consultum and each senatus auctoritas was transcribed into a final document by the presiding magistrate.
Total RNA (300 ng) was reverse transcribed in a final volume of 21 µl with 50 ng of random hexamers.
The RNA was reverse transcribed in a final volume of 20 µL using 15 U AMV Reverse Transcriptase, 40 U RNaseOUT™ (Invitrogen), 10 mM dithiothreitol (DTT), 4 µL 5×First-Strand buffer, at 42°C for 50 min, and then stopped by heating at 70°C for 15 min. The RT mixture was incubated with 2 U Ribonuclease H (Invitrogen) at 37°C for 20 min and the cDNA stock stored at −20°C.
Total RNA (300 ng) was reverse transcribed in a final volume of 21 μl with 50 ng of random hexamers.
For this purpose, 500 ng total RNA were reverse transcribed in a final volume of 100 μl.
For real time PCR (qRT-PCR) analysis three independent RNA preparations from each cell lines were reverse transcribed in a final volume of 10 μl.
Three μg of RNA were reverse transcribed in a final volume of 50 μL using the High Capacity cDNA Archive Kit with random primers (Applied Biosystems).
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CEO of Professional Science Editing for Scientists @ prosciediting.com