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Three different chimeric constructs were prepared to verify the localisation of the full length protein (pG2HPLF1-YFP) and the role of the first eleven amino acids at its N-terminus in the final targeting of HPLF (pG2HPLF2-YFP and pG2HPLF3-YFP).
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The complete nucleotide sequence of the final targeting vector was confirmed by DNA sequencing.
Forward and reverse DNA sequencing and restriction enzyme digests confirmed the integrity of the final targeting construct.
Finally, PUMs also bind diverse messages that are final targets of these signaling pathways, such as transcription factors that induce expression of angiogenic modulators or regulate cell proliferation or survival (Jun, STAT1, TCF4, TCF7L2).
Wiggins' final target of the season will be the world time trial championships in Florence on 25 September.
Law enforcement officials would not disclose the final targets of their investigation or say whether the names of steroid customers would ever be revealed.
And which, of the many women in the story, would you mark down as the final target of Anna Jessica Liedbergg), the smiling in-house lesbian?
Their last redoubts, General Franks's field commanders told him on Monday, were the final targets of the American-led military campaign.
There I learned that if my final target of £150,000 is reached, and along with the gift aid, it will fund nearly 3,000 nights in a refuge for a family.
As a result, industry will have a powerful tool to produce safer chemicals, which is the final target of REACH.
The final target of training is to produce 24 acoustic models (i.e., 12 for each chord type).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com