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Washington further insists that all final targeting decisions are made by the coalition.
The final targeting construct was designated pLoxpneoIng2 (Fig. S1a).
The final targeting construct is diagrammed in Figure 1A.
The final targeting vector γ/εP121L-GFP-p1002 is shown schematically in Fig. 1B.
The final targeting construct was linearised, electroporated into R1/E 129 mouse stem cells grown on feeders and selected using HygB.
The final targeting vector (Figure 1) was linearized with SacII at a unique site in the vector backbone.
The final targeting vector was electroporated into C57BL/6 ES cells.
The final targeting vector was linearized by digestion with XhoI and electroporated in 129Sv cells.
The final targeting vector was characterized by restriction site analyses and afterwards partially sequenced.
The complete nucleotide sequence of the final targeting vector was confirmed by DNA sequencing.
Forward and reverse DNA sequencing and restriction enzyme digests confirmed the integrity of the final targeting construct.
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