Sentence examples for final stringent from inspiring English sources

This brings the final stringent motif to [VLM][PGED][SAGEKL]{G}X 0,1)[VAIML][ESPQLK][GKEY][VLA][ED] with 325 protein hits in human.

As a final stringent analysis of Huv-iPS cell pluripotency, we evaluated the potential of each Huv-iPS cell line to differentiate into the three embryonic germ layers in vitro and in vivo.

Immunoprecipitation assays were carried out on myoblast extracts in RIPA buffer at 4°C overnight, following extensive washes and a final stringent wash in RIPA buffer containing 0.6 M LiCl2, bound proteins were analysed by SDS-PAGE and western blotting as above.

RNAs were transferred to Hybond N+ membranes followed by hybridisation at 65°C in modified Church and Gilbert buffer (7% SDS, 10 mM EDTA, 0.5 M sodium phosphate pH 7.2) and washed at 65°C in 2 × standard saline citrate (SSC; 1 × = 150 mM sodium chloride and 15 mM sodium citrate, pH 7.0), 0.1% SDS (2 × 15 min), with a final stringent wash in 0.1 × SSC, 0.1% SDS (2 × 15 min).

The strategy where the final list of stringent CNVRs was found by both algorithms has been recommended previously in order to reduce the false discovery rate [ 25].

Nevertheless, the final rules "are extremely stringent.

The final EA product with stringent specifications of 0.1 wt% EtOH and 0.005 wt% AA impurities can be achieved with this proposed overall control strategy despite feed flow rate and feed composition disturbances.

Minimizing driver beam interference and irradiation of the final focus magnets places stringent requirements upon the surface smoothness of the stationary liquid sheets that shield the heavy-ion driver array.

After final rinsing with non-stringent wash buffer, the microarrays were stained with 1X Stain buffer (100 mM MES, 1 M Na+, 0.05% Tween20, 50 mg/ml of BSA, and 1 mg/ml of Cy3-streptavidin) at room temperature for 25 min. After the stain buffer was removed, the microarrays were rinsed with non-stringent wash buffer and immediately dried under argon gas.

Negotiators in Kigali conceded that the final deal was much less stringent than some had originally hoped.

All these complicated factors pose a challenge to precisely control anodization process, especially when there is a stringent requirement on final membrane thickness.