Sentence examples for final soak from inspiring English sources

The final soak contained the mother liquor containing sodium pyruvate (100 m m) and 25%% (v/ v) PEG 400.

There was a final soak; 72°C for 5 min. Myoglobin intron II was amplified using the primer Myo2 in combination with Myo3F or Myo3 [ 31, 49].

The amplification condition was 50°C for 2 min; 95°C for 10 min, followed by 40 cycles of 95°C for 15 sec and 59°C for 1 min; a final soak at 4°C was also incorporated.

PCR conditions for the multiplex assay are as follows: denaturation for 11 min at 95°C, amplification for 30 cycles of 45 sec at 94°C, 2 min at 59°C, and 1 min at 72°C, extension for 60 min at 60°C, and a final soak at 25°C.

Samples were treated with 2 μl of ExoSap-IT™ (USB Corporation) per 5 μl of PCR product to remove unincorporated primers and dNTPs and run on a thermocycler with the following conditions: 90 min at 37°C, 20 min at 80°C to inactivate the enzymes, and a final soak at 25°C.

PCR for partial nifH gene was performed under the following conditions: 1 cycle at 94°C for 4 min, 25 cycles at 94°C for 30 sec, 54°C for 30 sec, 68°C for 30 sec, 1 cycle at 68°C for 7 min, and a final soak step at 4°C.

As Mr. Sehnalik starts up a sing-along of old Czech folk on guitar, I'm handed a final soak-up-the-alcohol snack ( skvarky)—a piece of bread smeared with lard and bits of fatty bacon.

As Mr Sehnalik starts up a sing-song of old Czech folk on guitar, I'm handed a final soak-up-the-alcohol snack ( skvarky) – a piece of bread smeared with lard and bits of fatty bacon.

As Mr Sehnalik starts up a sing-song of old Czech folk on guitar, I'm handed a final soak-up-the-alcohol snack ( skvarky)—a piece of bread smeared with lard and bits of fatty bacon.

Cycling conditions used an initial denaturation of 96 °C for 2 min, 30 cycles of 94 °C for 10 s, 61 °C for 1 min, and 30 s at 72 °C followed by a 20 min hold at 60 °C and a final 4 °C soak using the max ramp rate on a MJ Research DNA Engine Tetrad 2. Separation of amplification products was performed on the Applied Biosystems 3730xl.

Briefly, mouse brains from 12 month old control wild-type and age-matched A53T α-Syn mutant mice were perfused with 4% PFA, and prepared in a sequential sucrose gradient, from 10% to a final 30% sucrose soak.