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Final reactions contained 5 μL of template and 15 μL of master mixture.
The same concentration of total DNA was used in the final reactions for all DNAs prepared from infected chicken caeca.
The final reactions were made to a total volume of 20 μl with Quantitect SyBr Green PCR kit (Qiagen).
Final reactions of 25 μl of illustra PuReTaq Ready-To-Go PCR beads (GE Health Biosciences, USA) were used in the PCR reaction according to the manufacturer's recommendations.
Glycosyltransferase enzymes catalyze the final reactions in the biosynthesis of SGAs, where different sugar moieties are added to the solanidine aglycone.
The final reactions contain 0.1 25 mM NaCl from the enzyme storage stocks, but these concentrations of NaCl do not have noticeable effects on enzyme rate.
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Finally, in Fig. 5c, there is the final reaction when she saw the circular menu.
Finally, acetone is reformed to produce hydrogen and carbon dioxide, which were the final reaction products.
And then there are those whose first, and final, reaction is "yuck".
A final conversion of greater than 99 percent is achieved after passage through the final reaction bed.
The final reaction network takes the form of Fig. 2.
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CEO of Professional Science Editing for Scientists @ prosciediting.com