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In p1, the final quantity of iron (A+) was added directly to the final dyebath B but in p2 and p3, it was applied in a separate dyebath due to substantial and problematic foam formation in p1.
Whatever the peptide dose (10, 30 and 100 µg), the final quantity of adjuvant Montanide ISA-700 or AS02A for each formulation was constant.
The pool of HEK293E cells expressing the HA-tagged RhCG was expanded at 37°C, 5% CO2 in Dulbecco's modified Eagle's medium-Glutamax I (GIBCO, Invitrogen) supplemented with 10% fetal calf serum to a final quantity of 2×108 cells.
The final quantity of solvent did not exceed 0.1%% of culture media for all experiments.
This made the final quantity of volume replacement in each animal equal to 120% of the SBV.
In B. emersonii, growth in light causes the nucleic acid content of the cells to increase more rapidly than in darkness and to reach a 28% higher final quantity of nucleic acids than dark-grown cells (Goldstein and Cantino 1962).
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Based on the known quantities of stable isotope internal standards, the content of the corresponding peptides in a sample can be calculated, and the final quantities of the corresponding proteins can be determined.
These were applied to sterile discs such that the final quantities of 1 on the discs were 1 ng, 10 ng, 50 ng, 100 ng, 1 μg, and 10 μg.
Replicate sets containing nubbins from different colonies (n = 3, including more than 300 polyps each) were supplemented with aliquots of sunscreens (at final quantities of 10, 33, 50, and 100 μL/L seawater) and compared with untreated systems (used as controls).
The microbial production of polyhydroxybutyrate (PHB) is a complex process in which the final quantity and quality of the PHB depend on a large number of process operating variables.
This step allowed for relative quantification of the input DNA level and final quantity as the number of viral copies/genome/cell.
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