Sentence examples for final promoter from inspiring English sources
The final promoter set included nearly 16,000 sequences.
All pDONR subclones were sequenced to confirm lack of mutations, and att recombination sites were sequenced in the final promoter:gene:3 ′ UTR constructs prior to microinjection.
Taking these two criteria into consideration, PfhuA1 in Strategy 1, performed similarly as PBAD, was designated as the final promoter candidate for the next experiments.
For each "TRAINING MATRIX", another matrix composed of background promoter regions, which were not included in both the "model-build set" and the "motif-prediction set" (see "Final promoter sets" in Methods section) of a PS was further formed.
In order to reduce the number of motif sampling rounds, the elimination of promoters that are less probable to maximize the ll-scores of the detected motifs is predicated, otherwise the screening procedure becomes time-exhaustive and possibly non converging to a final qualified promoter set, with an increasing number of sequences embedded in the qualified promoter dataset.
A California jury decided on Wednesday that Michael Jackson's final concert promoter, A.E.G.
We further used the H3K4me3/me1 ratio to define final enhancer and promoter selections (Pekowska et al., 2011; de Almeida et al., 2011; Li et al., 2012; Natoli and Andrau, 2012).
In the final PCR, hapX promoter DNA linked to venus coding sequence was fused to hapX coding sequence including the 3′ terminator region with primers hapXtrunc-f/hapX-r (PCR5, 3.5 kb; template PCR3 & PCR4).
After rapid mixing at the indicated temperature (37°C or 25°C), the final concentrations were: promoter DNA, 0.3 nM; RNAP, 2 to 150 nM in binding buffer (20 mM HEPES, pH 8.0, 100 mM K-Glutamate, 10 mM MgCl2, 1 mM DTT).
Finally, transcription from the promoter within the final GUSBP1 intron can drive expression of both NAIP1 and NAIP2, and potentially gives rise to 100 kDa (NAIP1) and 130 kDa (NAIP2) proteins, respectively.
