Sentence examples for final precipitation from inspiring English sources

However, the longer reaction times and requirement of additional steps for the final precipitation of tetrabromobisphenol A are the major drawbacks associated with these methods.

Bacterial DNA was extracted from aliquots of feces, and after the final precipitation, DNA was resuspended in 150 µL of TE buffer, and stored at −20°C for further analysis, as previously described [26].

The final precipitation was analysed by Western blotting.

After a final precipitation step, all the RNAs were dissolved in nuclease-free water.

Take care not to over-dry the DNA pellets at this point (or at the final precipitation, step 18) or they will become very difficult to resuspend.

Total RNA was isolated by use of Trizol reagent (Invitrogen, Carlsbad, CA) followed by double precipitation with isopropanol and final precipitation with 5 M lithium chloride [ 45].

Subsequently, 30 mL 1 M oxalic acid solution was heated to boiling with magnetic stirring and added into the above solution slowly under constant stirring to form a final black precipitation, and then cooled by ice-water mixture.

The DNA was first precipitated in a sodium acetate/ethanol solution, and then purified by phenol/chloroform extraction, followed by the final ethanol precipitation.

Subsequently, the pools were purified by an additional phenol-chloroform extraction procedure including a final ethanol precipitation, because the pilot studies had also demonstrated that this treatment strongly improves the results of the array analyses.

To summarise, the following principle procedures were followed: Total RNA was isolated from freeze-dried tissues using the Trizol procedure (Chomczynski and Sacchi, 1987) incorporating a final LiCl2 precipitation.

Methods employing slightly quicker extraction of DNA using a 75 cm culture flask via short ultracentrifugation or concentration of the virus using a membrane filter followed by subsequent treatment with proteinase K to hydrolyze the viral proteins with final ethanol precipitation of viral DNA have been reported [ 18, 19].